Immobilization on Camelid Antibodies for therapeutic Apheresis and Dialysis support

Department of Science & Technology

Project Description

This project aims at utilizing camelide antibodies, which are much smaller then human antibodies, to, together with surface specialists at the Austrian Institute of Technologies (AIT), create a surface with high density of immobilized Camelid antibodies and therefore better binding affinity of target molecules or molecule groups.

Those surfaces are meant to be applied in products for therapeutic apheresis, together with Fresenius Medical Care, to achieve improved purification of and removal of specific harmful substances from patient blood.

Further Information
Status: Completed
Project ID : 1208
Sponsor :
Land NÖ

Project Leader
Prof.(FH) Priv.-Doz. Mag. Dr. Harald Hundsberger
Head of Institute Biotechnology / Programme Director Medical and Pharmaceutical Biotechnology
Prof.(FH) Priv.-Doz. Mag. Dr. Harald Hundsberger
Head of Institute Biotechnology / Programme Director Medical and Pharmaceutical Biotechnology
Institute Biotechnology
+43 2732 802 521
[email protected]
IMC Campus KremsIMC Campus Trakt G
Recombinant Protein Expression, Cell Culture Models, Peptide Engineering,
Instrument Development
Medical and Pharmaceutical Biotechnology
Bachelor of Science in Engineering / full-time
Medical and Pharmaceutical Biotechnology
Master of Science in Engineering / full-time
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Colleselli, K., Ebeyer-Masotta, M., Neuditschko, B., Stierschneider, A., Pollhammer, C., Potocnjak, M., Hundsberger, H., Herzog, F., Wiesner, C. (2023): Beyond Pattern Recognition: TLR2 Promotes Chemotaxis, Cell Adhesion, and Migration in THP-1 Cells. Cells, 12(10): 1425.
Doi: https://doi.org/10.3390/cells12101425
Stierschneider, A., Neuditschko, B., Colleselli, K., Hundsberger, H., Herzog, F., & Wiesner, C. (2023): Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells. Cells, 12(697).
Doi: https://doi.org/10.3390/cells12050697
Lucas, R., Hadizamani, Y., Enkhbaatar, P., Csanyi, G., Caldwell, R. W., Hundsberger, H., Sridhar, S., Lever, A. A., Hudel, M., Ash, D., Ushio-Fukai, M., Fukai, T., Chakraborty, T., Verin, A., Eaton, D. C., Romero, M., & Hamacher, J. (2022): Dichotomous Role of Tumor Necrosis Factor in Pulmonary Barrier Function and Alveolar Fluid Clearance. Frontiers in Physiology, 12: 793251.
Doi: https://doi.org/10.3389/fphys.2021.793251
Weitzenböck, HP., Gschwendtner, A., Wiesner, C., Depke, M., Schmidt, F., Trautinger, F., Hengstschläger, M., Hundsberger, H., Mikula, M. (2022): Proteome analysis of NRF2 inhibition in melanoma reveals CD44 up-regulation and increased apoptosis resistance upon vemurafenib treatment. Cancer Medicine, 11(4): 956-967.
Doi: https://doi.org/10.1002/cam4.4506
Stierschneider, A., Grünstäudl, P., Colleselli, K., Atzler, J., Klein, C., Hundsberger, H., Wiesner, C. (2021): Light-Inducible Spatio-Temporal Control of TLR4 and NF-κB-Gluc Reporter in Human Pancreatic Cell Line. International Journal of Molecular Sciences, 22(17): 9232.
Doi: https://doi.org/10.3390/ijms22179232
Hundsberger, H., Stierschneider, A., Sarne, V., Ripper, D., Schimon, J., Weitzenböck, H. P., Schild, D., Jacobi, N., Eger, A., Atzler, J., Klein, C. T., & Wiesner, C. (2021): Concentration-Dependent Pro- and Antitumor Activities of Quercetin in Human Melanoma Spheroids: Comparative Analysis of 2D and 3D Cell Culture Models. Molecules (Basel, Switzerland), 26(3): 717.
Doi: https://doi.org/10.3390/molecules26030717
Kinslechner, K., Schütz, B., Pistek, M., Rapolter, P., Weitzenböck, H. P., Hundsberger, H., Mikulits, W., Grillari, J., Röhrl, C., Hengstschläger, M., Stangl, H., & Mikula, M. (2019): Loss of SR-BI Down-Regulates MITF and Suppresses Extracellular Vesicle Release in Human Melanoma. International journal of molecular sciences, 20(5): 1063.
Doi: https://doi.org/10.3390/ijms20051063
Jacobi, N., Seeboeck, R., Hofmann, E., Schweiger, H., Smolinska, V., Mohr, T., Boyer, A., Sommergruber, W., Lechner, P., Pichler-Huebschmann, C., Önder, K., Hundsberger, H., Wiesner, C., & Eger, A. (2017): Organotypic three-dimensional cancer cell cultures mirror drug responses in vivo: lessons learned from the inhibition of EGFR signaling. Oncotarget, 8(64): 107423–107440.
Doi: https://doi.org/10.18632/oncotarget.22475
Hundsberger, H., Önder, K., Schuller-Götzburg, P., Virok, D. P., Herzog, J., & Rid, R. (2017): Assembly and use of high-density recombinant peptide chips for large-scale ligand screening is a practical alternative to synthetic peptide libraries. BMC genomics, 18(1): 450.
Doi: https://doi.org/10.1186/s12864-017-3814-3
Jacobi, N., Smolinska, V., Seeboeck, R., Stierschneider, A., Klein, C., Hofmann, E., Wiesner, C., Mohr, T., Oender, K., Lechner, P., Kaiser, H., Hundsberger, H., Eger, A. (2017): 3D Anti-Cancer drug discovery models: A promising approach for precision medicine. In IMC Fachhochschule Krems GmbH (Hrsg.), Online-Tagungsband FHK Forschungsforum 2017. Krems: FFH.
Hundsberger, H., Koppensteiner, A., Hofmann, E., Ripper, D., Pflüger, M., Stadlmann, V., Klein, C. T., Kreiseder, B., Katzlinger, M., Eger, A., Forster, F., Missbichler, A., & Wiesner, C. (2017): A Screening Approach for Identifying Gliadin Neutralizing Antibodies on Epithelial Intestinal Caco-2 Cells. SLAS discovery : advancing life sciences R & D, 22(8): 1035–1043.
Doi: https://doi.org/10.1177/2472555217697435
Volk, K., Breunig, S. D., Rid, R., Herzog, J., Bräuer, M., Hundsberger, H., Klein, C., Müller, N., & Önder, K. (2017): Structural analysis and interaction studies of acyl-carrier protein (acpP) of Staphylococcus aureus, an extraordinarily thermally stable protein. Biological chemistry, 398(1): 125-133.
Doi: https://doi.org/10.1515/hsz-2016-0185
Schütz, B., Koppensteiner, A., Schörghofer, D., Kinslechner, K., Timelthaler, G., Eferl, R., Hengstschläger, M., Missbichler, A., Hundsberger, H., & Mikula, M. (2016): Generation of metastatic melanoma specific antibodies by affinity purification. Scientific reports, 6: 37253.
Doi: https://doi.org/10.1038/srep37253
Al-Harthy, T., Zoghaib, W. M., Pflüger, M., Schöpel, M., Önder, K., Reitsammer, M., Hundsberger, H., Stoll, R., & Abdel-Jalil, R. (2016): Design, Synthesis, and Cytotoxicity of 5-Fluoro-2-methyl-6-(4-aryl-piperazin-1-yl) Benzoxazoles. Molecules, 21(10): 1290.
Doi: https://doi.org/10.3390/molecules21101290
Al-Harthy, T., Abdel-Jalil, R., Zoghaib, W., Pflüger, M., Hofmann, E., & Hundsberger, H. (2016): Design and synthesis of benzothiazole schiff bases of potential antitumor activity. Heterocycles, 92(7): 1282-1292.
Doi: https://doi.org/10.3987/COM-16-13471
Hofmann, E., Seeboeck, R., Jacobi, N., Obrist, P., Huter, S., Klein, C., Oender, K., Wiesner, C., Hundsberger, H., & Eger, A. (2016): The combinatorial approach of laser-captured microdissection and reverse transcription quantitative polymerase chain reaction accurately determines HER2 status in breast cancer. Biomarker research, 7(4): 8.
Doi: https://doi.org/10.1186/s40364-016-0062-7
Jacobi, N., Smolinska, V., Stierschneider, A., Klein, C., Oender, K., Lechner, P., Kaiser, H., Hundsberger, H., Eger, A. (2016): Development of organotypic cancer models for the identification of individualized cancer therapies. In FH des BFI Wien (Hrsg.), Online-Tagungsband FHK Forschungsforum 2016. Wien: FFH.
Herzog, J., Rid, R., Wagner, M., Hundsberger, H., Eger, A., Bauer, J., & Önder, K. (2015): Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways. BMC research notes, 8: 785.
Doi: https://doi.org/10.1186/s13104-015-1783-7
Kreiseder, B., Holper-Schichl, Y. M., Muellauer, B., Jacobi, N., Pretsch, A., Schmid, J. A., de Martin, R., Hundsberger, H., Eger, A., & Wiesner, C. (2015): Alpha-catulin contributes to drug-resistance of melanoma by activating NF-κB and AP-1. PloS one, 10(3): e0119402.
Doi: https://doi.org/10.1371/journal.pone.0119402
Pretsch, A., Nagl, M., Schwendinger, K., Kreiseder, B., Wiederstein, M., Pretsch, D., Genov, M., Hollaus, R., Zinssmeister, D., Debbab, A., Hundsberger, H., Eger, A., Proksch, P., & Wiesner, C. (2014): Antimicrobial and anti-inflammatory activities of endophytic fungi Talaromyces wortmannii extracts against acne-inducing bacteria. PloS one, 9(6): e97929.
Doi: https://doi.org/10.1371/journal.pone.0097929
Rid, R., Hundsberger, H., & Onder, K. (2014): Compound screening and transcriptional profiling in human primary keratinocytes: a brief guideline. Methods in molecular biology, 1195: 99-109.
Doi: https://doi.org/10.1007/7651_2013_50
Kapuścik, A., Hrouzek, P., Kuzma, M., Bártová, S., Novák, P., Jokela, J., Pflüger, M., Eger, A., Hundsberger, H., Kopecký, J. (2013): Novel Aeruginosin-865 from Nostoc sp. as a potent anti-inflammatory agent. Chembiochem : a European journal of chemical biology, 14(17): 2329-2337.
Doi: https://doi.org/10.1002/cbic.201300246
Rid, R., Herzog, J., Maier, R. H., Hundsberger, H., Eger, A., Hintner, H., Bauer, J. W., Onder, K. (2013): Real-time monitoring of relative peptide-protein interaction strengths in the yeast two-hybrid system. Assay and drug development technologies, 11(4): 269-275.
Doi: https://doi.org/10.1089/adt.2012.496
Rid, R., Wagner, M., Maier, C. J., Hundsberger, H., Hintner, H., Bauer, J. W., & Onder, K. (2013): Deciphering the calcitriol-induced transcriptomic response in keratinocytes: presentation of novel target genes. Journal of molecular endocrinology, 50(2): 131–149.
Doi: https://doi.org/10.1530/JME-11-0191
Kreiseder, B., Orel, L., Bujnow, C., Buschek, S., Pflueger, M., Schuett, W., Hundsberger, H., de Martin, R., Wiesner, C. (2013): α‐Catulin downregulates E‐cadherin and promotes melanoma progression and invasion. International journal of cancer, 132(3): 521-530.
Doi: https://doi.org/10.1002/ijc.27698
Pflüger, M., Kapuscik, A., Lucas, R., Koppensteiner, A., Katzlinger, M., Jokela, J., Eger, A., Jacobi, N., Wiesner, C., Hofmann, E., Onder, K., Kopecky, J., Schütt, W., Hundsberger, H. (2013): A combined impedance and AlphaLISA-based approach to identify anti-inflammatory and barrier-protective compounds in human endothelium. Journal of biomolecular screening, 18(1): 67-74.
Doi: https://doi.org/10.1177/1087057112458316
Khare, V., Lyakhovich, A., Dammann, K., Lang, M., Borgmann, M., Tichy, B., Pospisilova, S., Luciani, G., Campregher, C., Evstatiev, R., Pflueger, M., Hundsberger, H., & Gasche, C. (2013): Mesalamine modulates intercellular adhesion through inhibition of p-21 activated kinase-1. Biochemical Pharmacology, 85(2): 234-244.
Doi: https://doi.org/10.1016/j.bcp.2012.10.026
Maier, C., Maier, R., Rid, R., Trost, A., Hundsberger, H., Eger, A., Hintner, H., Bauer, J., Onder, K. (2012): PIM-1 kinase interacts with the DNA binding domain of the vitamin D receptor: a further kinase implicated in 1,25-(OH)2D3 signaling. BMC molecular biology, 13: 18.
Doi: https://doi.org/10.1186/1471-2199-13-18
Amatschek, S., Lucas, R., Eger, A., Pflueger, M., Hundsberger, H., Knoll, C., Grosse-Kracht, S., Schuett, W., Koszik, F., Maurer, D., Wiesner, C. (2011): CXCL9 induces chemotaxis, chemorepulsion and endothelial barrier disruption through CXCR3-mediated activation of melanoma cells. British journal of cancer, 104(3): 469-479.
Doi: https://doi.org/10.1038/sj.bjc.6606056
Hundsberger, H., Verin, A., Wiesner, C., Pflüger, M., Dulebo, A., Schütt, W., Lasters, I., Männel, D., Wendel, A., Lucas, R. (2008): TNF: a moonlighting protein at the interface between cancer and infection. Frontiers in Bioscience, 13: 5374-86.
Doi: https://doi.org/10.2741/3087
Hundsberger, H., Verin, A., Wiesner, C., Pflüger, M., Dulebo, A., Schütt, W., Lasters, I., Männel, D. N., Wendel, A., & Lucas, R. (2008): TNF: a moonlighting protein at the interface between cancer and infection. Frontiers in bioscience : a journal and virtual library, 13: 5374–5386.
Doi: https://doi.org/10.2741/3087
Wiesner, C., Pflueger, M., Kopecky, J., Stys, D., Entler, B., Lucas, R., Hundsberger, H., Schuett, W. (2008): Implementation of ECIS technology for the characterization of potential therapeutic drugs that promote wound-healing. GMS Krankenhaushygiene interdisziplinar, 3(1).
Wiesner, C., Pflüger, M., Kopecky, J., Stys, D., Entler, B., Lucas, R., Hundsberger, H., Schütt, W. (2008): Implementation of ECIS technology for the characterization of potential therapeutic drugs that promote wound-healing. GMS Krankenhaushygiene Interdisziplinär, 3(1): Doc05.
Wiesner, C., Lucas, R., Pflueger, M., Kleber, C., Kopecky, J., Stys, D., Entler, B., Hundsberger, H., Atzler, J., Hrouzek, P., Lukesova, A., Schuett, W., (2007): Endothelial Cell-Based Methods for the Detection of Cyanobacterial Anti- Inflammatory and Wound-Healing Promoting Metabolites. Drug Metabolism Letters, 1(4): 254-260.
Doi: https://doi.org/10.2174/187231207783221385
Lucas, R., Hundsberger, H., Pflueger, M., Fischer, B., Morel, D., Braun, C., Wendel, A., Chakraborty, T., Schuett, W., Wiesner, C., Hamacher, J. (2007): The Tumor Necrosis Factor-Derived TIP Peptide: A Potential Anti-Edema Drug. Letters in Drug Design & Discovery, 4(5): 336-340.
Doi: https://doi.org/10.2174%2F157018007780867816

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Immobilization on Camelid Antibodies for therapeutic Apheresis and Dialysis support

Department of Science & Technology

Project Description

Prof.(FH) Priv.-Doz. Mag. Dr. Harald Hundsberger